A selective method for sequential splitting of O- and N-linked glycans from N,O-glycoproteins. - PDF Download Free (2024)

Carbohydrate Research, 199 (1990) 61-76 Elsevier Science Publishers B.V., Amsterdam

67 - Printed in The Netherlands

A selective method for sequential splitting of O- and Nlinked glycans from N,O-glycoproteins Leonid M. Likhosherstov, K. Kochetkov

Olga S. Novikova,

Varvara

A. Derevitskaya,

N. D. Zelinsky Institute oJOrganic Chemistry, Academy of Sciences of the U.S.S.R.. (Received

May 25th, 1989; accepted

for publication.

and Nikolay

Moscow (U.S.S.R.)

July 21st, 1989)

ABSTRACT O-Linked alkaline sodium N-glycosylamide

oligosaccharides

from N,O-glycoproteins

were selectively

splitt off by treatment

NaBH, and 5OmM NaOH, 16 h, 507, was inhibited by addition of 5%1hM cadmium EDTA’Na,, as shown by treatment of model compounds and several glycoproteins specific glycoproteins

H and B, fetuin, and asialofetuin).

developed procedure

for the release of the N-linked oligosaccharide

sequential,

with

borohydride in the presence of cadmium salt. The side reaction of reductive cleavage and peptide bonds, observed under standard conditions of splitting of O-linked chains

This treatment,

in combination

acetate and 5-IOmM (ovomucoid, groupwith the previously

chains by lithium borohydride,

selective cleavage of 0-, and then N-linked oligosaccharides

of (M

from N,O-glycoproteins

allows a

by chemical

methods.

INTRODUCTION

Selective release of 0- and N-linked carbohydrate chains from N,O-glycoproteins is an important problem which has not been solved completely yet. The only known method’ is based on the selective cleavage of the N-glycosylamide bonds by an enzyme, peptide-N4-(N-acetyl-P-D-glucosaminyl)asparagine amidase with subsequent release of O-linked oligosaccharides (O-OS) by treatment with alkaline sodium borohydride. Chemical methods for the splitting of 0- and N-linked carbohydrate chains are rather unspecific. Hydrazinolysis of N,O-glycoproteins, which is used to relase N-linked oligosaccharide chains (N-OS), results in a substantial splitting of O-OS (up to 40%), followed by their partial degradation2. Alkaline sodium borohydride treatment of N,O-glycoproteins gave O-OS in good yields, but is accompanied with a simultaneous release of N-OS in lO-30% yields ‘-’ due to the reductive cleavage of the N-glycosylamide bond with sodium borohydride6. It is known7-9 that the specificity of reduction of various functional groups with sodium borohydride in alcoholic solution may be modified by addition of inorganic salts. Thus, we studied the influence of some salts containing the cations Co’+, Ni2+, Pb2+, Cu2+, Znzf, Ba2+, and Cd’+ on the splitting off of O-OS from N,O-glycoproteins under standard conditions” (M sodium borohydride, 50mM sodium hydroxide, 16 h, SO’). It was found that addition of Cd2+ salts significantly increased the selectivity of the reaction”. We report herein a new procedure for the selective liberation of O-OS, and 0008-62 15/90/S 03.50

@ 1990 Elsevier Science Publishers

B.V

68

L.M.

propose a method for the sequential glycoproteins, based on a combination

splitting of 0-, and then N-OS of this method with the recently

method

for the release of N-OS by lithium

RESULTS

AND I~ISCUSSION

borohydride

et al.

LIKHOSHERSTOV

from N.Odescribed”

treatment.

The influence of Cd’+ salt upon reductive cleavage of N-glycosylamide and peptide bonds with alkaline sodium borohydride was tested on 2-acetamido-4-O-(2-acetamido-2-deoxy-j’-u-glucopyranosyl)1-N-(4-L-aspartoyl)-2-deoxy-P_D-glucopyranosylamine (1) and glycylglycine as model compounds. The mixture obtained after treatment of 1 with aqueous M sodium borohydridee50mM sodium hydroxide for 16 h at 50’ contained, in addition to the starting glycosylamino acid 1(80%). 2-amino-4-hydroxybond), butyric acid (3, - 15%; the product of reductive cleavage of the N-glycosylamide and aspartic acid (4,- 3%; the product of alkaline hydrolysis). The reduced disaccharide 5 (18%) was also present; it was formed from the oligosaccharide released by alkaline hydrolysis of glycosylamine 2, the initial product of the reductive cleavage of the N-glycosylamide bond (2 is stable under the conditions of the reaction with lithium borohydride”). After treatment of glycopeptide 1 with alkaline sodium borohydride containing cadmium sulfate (from 0.1 up to ImM), starting 1 remained mainly intact, small proportions (3%) of 4 and 5 were detected, and the product of reductive cleavage 3 was not found. Analogous results were obtained with glycylglycine which gave 2-aminoethanol in a yield of 9% (the product of reductive cleavage) only after treatment with alkaline sodium borohydride without cadmium sulfate. These results demonstrated that the presence of a cadmium salt in alkaline sodium borohydride solution leads to inhibition of the reductive cleavage of N-glycosylamide and peptide bonds.

ii Q ’

HOCHi

o

NHCCH2CHC0,H

OH

NH2

RO

I

NoBH4

4+5

+

Cd’

NoBHq

+

HOCHzCH*CHCO*H

t

HOZCCH;CHCO,H

I

I

NH2

NH?

3

4

SEQUENTIAL

TABLE

SPLITTING

OF GLYCANS

69

FROM GLYCOPROTEINS

I

Yields of olygosaccharides

after alkaline

borohydride

treatment

of glycoproteins.

Reagents Glycoprotein

Yields (%) of

LiBH,

LiOH

N&H

04

@MI

(M)

2

Cd’+

Time

Temp.

@I

(“1

25 25

16 16 16 16 10 5 5

50 50 50 60 60 45 45

25

5

45

5c-55

16 16 16 10

50 50 60 60

15-20 34 4.3 3.5

16 16 16 16 10 5

50 50 60 60 60 60

Fetuin or asialofetuin

Glycopeptide fraction”

NaOH

Ovomucoid

GSG H or GSG B

” From fetuin or asialofetuin

4 w4

1

50

1

51

+

2 2 2

21 27 27

+ +

1 1 2 2

50 51 2-I 21

1 1 2 2 2 2

50 57 21 21 27 21

after NaBH,-Cd2+

+ + +

+ + + + treatment.

O-OS

N-OS

5@55 67-73 48-53 85-90 75-80 32 l&l5

15-20 3-4 23-25 7-8 3.54 41 6lS63

61-66 70-75 65 85-90 78-83 65

h Traces.

Subsequently, we investigated the influence of Cd*+ salt in alkaline sodium borohydride on the splitting of O-OS and N-OS, and the cleavage of the peptide chain of glycoproteins by use of fetuin and asialofetuin (containing both 0- and N-linked chains), ovomucoid (containing N-linked chains), and group-specific glycoproteins (GSG) H and B (containing O-linked chains). Inhibition of the reductive cleavage of the amide bonds could be achieved by the use of a lo-fold higher concentration of Cd2+ salt. The addition of tetrasodium ethylenediaminetetraacetate (EDTA.Na,) was found to be necessary to prevent the precipitation ofcadmium hydroxide from the alkaline solution. The chelating agent showed no influence on the splitting of 0- and N-OS with alkaline sodium borohydride in the absence of Cd*+ salt. The treatment (16 h, 50”) of fetuin, asialofetuin, and ovomucoid with a solution of M sodium borohydride in 57 mM sodium hydroxide containing 6mM cadmium acetate and 6mM EDTA.Na, led to liberation of O-OS (70%) from fetuin or asialofetuin, and only a minor release of N-OS (34%) as compared to 1520% in the absence of Cd*+ salt (see Table I). That the splitting of N-OS was due to alkaline hydrolysis of the N-glycosylamide bond was confirmed by control experiments in which ovomucoid and fetuin were treated with sodium hydroxide alone. No reductive cleavage of the peptide chain was observed upon alkaline treatment

L.M. LIKHOSHERSTOV

et Ul.

-,

,

I 0

20

. 40

-y-60 Elutlon

, 80 volume

100

120

(mi)

Fig. I. Gel chromatography on a Sephadex G-75 column (I .55 x 62 cm) in 4mM NH,OH of: (I) fetuin: (2) fetuin treated with M NaBH, and 50 mM NaOH for 16 h at SO’ : and (3) fetuin treated with M NaBH, at 57mMNaOH and 6mM cadmium acetate 6mM EDTA,Na, solution for 16 h SO ‘. Fractions were analyzed after hydrolysis (4~ HCI, 16 h. 100 ‘) with an amino acid analyzer (buffer C). Absorbance at 570 nm after ninhydrin reactlon gave a sum of neutral amino acids for (I), (2), and (3); and 2-amino-2-deoxy-u-glucose (GlcN) for (2). The arrows show the elution volume of reference compounds: (a) Dextran Blue: (b) triglycylglycinc.

with sodium borohydrideeCd* ofglycoproteins as well. This conclusion could be drawn from gel chromatography data. Thus, gel filtration on Sephadex G-75 resulted in almost identical elution curves for fetuin before and after treatment with alkaline sodium borohydride+Cd”, whereas treatment without Cd” produced a considerable depolymerization with complete absence of high-molecular-weight products (Fig. 1). The depolymerization was accompanied by a decrease in content of several amino acids (Table II). For example, the proportions of aspartic and glutamic acids. and glycine in GSG H diminished by 37. 46% after treatment with akaline sodium borohydride, whereas no change was observed upon treatment with alkaline sodium borohydrideCd’+. Increase in alanine content and formation of 2-aminobutyric acid in both cases indicated the partial reduction by sodium borohydride of 2-aminopropenoic and 2-amino-2-butenoic acid residues that were formed upon /?-elimination of O-OS from serine and threonine residues. The addition of Cd’ ’ salt to alkaline sodium borohydride not only increased the selectivity of &OS splitting, but also resulted in higher yields of oligosaccharide alditols (Table 1). This increase probably resulted from the inhibition of the cleavage of

71

SEQUENTIAL SPLITTING OF GLYCANS FROM GLYCOPROTEINS

TABLE II Amino acid comoosition

of GSG H after treatment

Amino acid

with various

reagents

Conditions

Aspartic acid Threonine Serine Glutamic acid Proline Glycine Alanine 2-Aminobutyricb

acid

A

B

c

62.5 12.9 10.7 63 76 54 136 41

100 25 19.5 91.5 92 97.5 204 14

100 24.5 22.5 100 88 95 201 19

“(A) M NaBH,, 50mM NaOH 16h, 50”; (B) M NaBH,, 57mM NaOH, 6mM EDTA.Na,, 6mM cadmium acetate, 16 h, 50”; and (C) 2~ NaBH,, 27mM NaOH, 6mM EDTA.Na,, 6mM cadmium acetate, 10 h, 60” (Percentage with respect to initial content). hFrom threonine in initial GSG H.

the peptide chain. In fact, 2-amino-1,3-propanediol and 2-amino-1,3-butanediol, the products of reductive cleavage of peptide bonds at serine and threonine residues, were identified among the amino alcohols formed by alkaline sodium borohydride treatment of fetuin. It may be expected that the reductive cleavage of the bonds of the 2-amino-3hydroxy acid residues bearing carbohydrate chains gives compounds 6 or 7, which are either stable against or rather resistant to B-elimination of O-OS. For determination of the influence of reductive cleavage of the peptide chain on the yield of O-OS, fetuin was treated with lithium borohydride, which produced a reductive cleavage of peptide bonds much more extensive than did sodium borohydride12. It was found that the increase in concentration of lithium borohydride (from 1.4 to 2M), and therefore of the velocity of the reductive cleavage led to a decrease in the yield of O-OS (from 32 to 12%; Table I). The O-linked oligosaccharides remaining unsplit (88% after 2M lithium borohydride and 45% after M sodium borohydride treatment) were found to be stable under mild alkaline conditions, and remained practically intact after repeated treatment with alkaline sodium borohydride or sodium borohydride-Cd’+. The inhibition by Cd’+ salt of the reductive cleavage of the peptide chain by alkaline sodium borohydride treatment opened the route to a more efficient procedure for the liberation of O-OS from glycoproteins. When fetuin or GSG were treated with a 2~ solution of sodium borohydride in 27mM sodium hydroxide containing 6mM EDTA.Na, and 6mM cadmium acetate for 16 h at 60”, O-OS were released in yields of CH,OH HO

CH20H

CH,OH

0 \L

o’Hl;...

NoBH4

HOQo~Hi:...

+

v CH20H

co--6 R

=

H

AcNH

AcNH

AcNH

or

CH3

HO~o~H~~

co--7

L.M. LIKHOSHERSTOV

72 85-90% (Table N-glycosylamide

et d.

I). Under these conditions, however the alkaline hydrolysis of the bond was more significant and N-linked chains (778%) were split off

from fetuin. For this reason, it seems advisable to use these conditions O-OS from 0-glycoproteins. To split of O-OS from N,O-glycoproteins

for release of in rather high

yields (7&80%) and sufficiently selectively, it is recommended to use alkaline M or 2M sodium borohydrideecd’ ’ (16 h, 50”; or 1Oh, 60”, respectively). It should be noted that under all conditions applied, including those described earlier”‘, N-deacetylation of 2-acetamido-2-deoxy-o-hexose residues was not more than 2 3% for GSG H or B, and was not detected for fetuin and asialofetuin. Selectivity of splitting of O-OS with alkaline sodium borohydrideeCd”’ and stability of the remaining 0 OS in glycoprotein towards the action of alkaline lithium borohydride suggested a procedure of stepwise release of 0-, and then &OS from N,O-glycoproteins. This procedure involves the treatment of N,O-glycoprotein with M or 2M sodium borohydride for 16 h at 50” or 10 h at 60”, respectively, followed by addition of acetic acid to pH 7-7.5, and separation of the partially deglycosylated glycoprotein and oligosaccharides as alditols by gel chromatography in dilute ammonium hydroxide (pH - 9.5). It should benoted that gel chromatography in acidicmedia or cation-exchange chromatography are not satisfactory owing to the irreversible adsorption of a significant part of the deglycosylated glycoprotein. To split off N-OS from the partially deglycosylated glycoprotein, this was treated with alkaline lithium borohydride in 70% aqueous tert-butyl alcohol, as described previously”. and N-OS were isolated in a yield of 50&55%. This procedure, developed for asialofetuin, may be applied also for highly sialylated fetuin. In this case, separation of &OS and N-glycopeptides on Dowex 5OW-X2 (H ’ ) cation-exchange resin was achieved at 5’ with 5% acetic acid as an eluent. It may be concluded that alkaline sodium borohydride-Cd’ + is the first selective reagent for splitting of nondegraded, O-linked oligosaccharides from 0- and N,O-glycoproteins. Use of this reagent in combination with the lithium borohydride method” makes possible the sequential. chemical from N,O-glycoproteins.

release of both 0- and N-linked

oligosaccharide

chains

EXPERIMENTAL

Genera/ methods. --. Fetuin

(Sigma),

(ref. 15) were used as model glycoproteins.

ovomucoid”, Asialofetuin

GSG H (ref. 14) and GSG B was obtained

by incubation

(1.5 h, 800) of fetuin in diluted formic acid (pH 2.1). The following reagent mixtures were employed: (A) 50mM NaOH and M NaBH,; (B) 57mM NaOH, M NaBH,, 6mM cadmium acetate and 6mM EDTA.Na,; and (C) 27mM NaOH, 2M NaBH,, 6mM cadmium acetate, and 6mM EDTA. Na,. Reactions were performed in quartz test-tubes (1 x 15cm or 0.7 x 10cm) or in Pyrex glass test-tubes (1.8 x 20cm) covered with Parafilm. Butanol (5% v/v) was added to the reaction mixtures containing NaBH, to prevent foaming. Yields of oligosaccharides were calculated from the quantitative determination of 2-amino-2-deoxy-o-galactose and -o-galactitol for O-OS or 2-amino-2-deoxy-D-glucase and -D-ghCitOl for N-OS.

SEQUENTIAL

SPLITTING

OF GLYCANS

FROM GLYCOPROTEINS

73

Analytical methods. - Amino acids, 2-amino-2-deoxyhexoses, and amino alcohols were determined, after hydrolysis with 4~ HCl(16 h, loo’), by use of a post-column ninhydrin reaction after separation with the amino acid analyzer Microtechna T-339, equipped with an Ostion LG ANB column (0.38 x 17 cm). The following buffers were used: (A) 0.2M sodium citrate-HCl (pH 3.25) at 63” (B) 0.35~ sodium citrate_HCl (pH 5.28) at 63”, (C’) borateI (pH 7.24) at 89”, and (D) 0.7M sodium citrate-HCl (pH 5.28) at 63”; at a flow rate of 16.8 mL .h-‘. 2-Amino-4-hydroxybutyric acid was determined without hydrolysis on an amino acid analyzer Biotronik LC 4010, equipped with an Aminex A-6 column (0.9 x 23 cm) eluted with buffer A. Preparation of a solution of cadmium acetate-EDTA.Na,. - A mixture of solutions of 0.1~ EDTA.Na, (4.5mL) and cadmium acetate (23 mg.mL-‘, 4.5mL) was titrated with 0.5111NaOH (N 1.7 mL) to pH 7-7.5. Treatment

of 2-acetamido-4-0-(2-acetamido-2-deoxy-8_D-glucopyranosyl)-l-N-

(1). (a). To a solution of 1 (0.33 mg) in water (0.35 mL), previously titrated with 0.1~ NaOH to pH 7.5-8, were added water (0.1 mL), 0.5M NaOH (O.O5mL), and NaBH, (19mg). After being incubated for 16 h at 50”, the mixture was cooled, diluted with water (2.5 mL), acidified with cont. HCl to pH - 1.5, and diluted with water to 7 mL. Amino acid analysis of an aliquot (1 mL) indicated a content of 2-amino-4-hydroxybutyric acid (3) and free aspartic acid (4) of 15 and 3%) respectively. The remaining solution was coevaporated with methanol (3 x 3 mL). The residue was hydrolyzed with 4M HCl to give 4 (80%, buffer A), 2-amino-2-deoxy-D-glucitol(9%, buffer C), and 2-amino-2-deoxy-D-glucose (90%, buffer C). (b). An analogous procedure was used for the treatment of 1 with alkaline NaBH, containing 0. I-1mM CdSO,. Free aspartic acid content was determined as 3% and 3 was not found. After hydrolysis, 4 (- 100%) 2-amino-2-deoxy-D-glucitol (- 3%), and 2-amino-2-deoxy-D-glucose (N 100%) were determined. (c). An analogous treatment of 1 with 50mM MaOH led to 4 (3%). Treatment of gfycylglycine. - Glycylglycine (0.6mg) was treated as described before. After treatment with alkaline NaBH,, 2-aminoethanol (buffer D, 9%) and glycine (buffer A, 13% without hydrolysis) were determined. After treatment with 50mM NaOH, or alkaline NaBH, and CdSO,, glycine was determined without hydrolysis in 6% or 4% vield, resoectivelv; 2-aminoethanol was not found. Treatment offetuin andasialofetuin. - (a). Analytical experiments. A solution of fetuin or asialofetuin (1.5 mg) in reagent A, B, or C (0.5 mL) was incubated for 16 h at 50” (for A and B), or 10 and 16 h at 60” (for C). The mixture was cooled, diluted with water (3.5 mL), and acidified with acetic acid to pH 6. The solution was stirred (30min) with Dowex 5OW-X2 (H+, 3 mL) cation-exchange resin, and the resin was filtered off and washed with water. The filtrate was concentrated to dryness with addition of methanol (3 x 5 ml), and the residue was hydrolyzed and analyzed for 2-amino-2deoxy-D-glucose, -o-galactose, -o-glucitol, and -D-galactitol. The resin was washed with M NH,OH (20mL), the filtrate was concentrated, the residue was hydrolyzed, and 2-amino-2-deoxy-D-glucose and -o-galactose were determined. The yields of O-OS and (4-L-aspartoyl)-2-deoxy-P-D-glucopyranosylamine”

L.M. LIKHOSHERSTOV

74 N-OS

(Table

I) were calculated

from the content

et d.

of 2-acetamido-2-deoxy-D-glucose

and -D-galactose in the starting fetuin or asialofetuin. Loss of 2-acetamido-2-deoxy-Dglucose owing to irreversible adsorption of glycopeptides on Dowex resin was as much as 25%. (h), Isolation oj’O-OS reagent

B. An aqueous 2-amino-2-deoxy-D-glucose

andglvcopeptidefructionsfrorn,~etuin

uf!fiertreatment with

solution (2mL) of fetuin (7.8 mg, containing 1.52 pmol of and 0.25 prnol of 2 amino-2-deoxy-I>-galactose) was treat-

ed with cadmium acetate-EDTA’Na, solution (0.43 mL), water (0.23 mL), 0.5~ NaOH (0.34 mL), NaBH, (114 mg), and butanol (0.15 mL) for 16 h at 50”. The mixture was cooled, diluted with water to 15 mL, acidified with acetic to pH 7, and concentrated to 3 mL. The solution was fractionated on a Sephadex G-50 column (1 .X x 85 cm) in 4mM NH,OH (pH _ 9.5) to give three fractions. Fraction I (KC,, < 0.15) contained 1.16 knnol (76%) of 2-amino-2-deoxy-n-glucose, 52,6 nmol (21%) of 2-amino-2-deoxy-I>-galactose, and a considerable proportion of amino acids. Fraction II (y,, 0.15 -0.3) contained 0.29 pmol(l9%) of 2 amino-2-deoxy-D-glucose, 9.3 nmol(O.61%) of 2-amino-2-deoxyD-&ICitOl, 12.5 nmol(5.1%) of 2-amino-2-deoxy-D-galactose, and amino acids. It was identified as a mixture ofglycopeptides and ofa small proportion of N-OS (3. I%). Both fractions were combined and concentrated to dryness, and the resulting glycopeptide fraction was used for liberation of N--OS. Fraction III (K,,. 0.3~.0.85) contained 0.183 /lmol(73%) of 2-amino-2-deoxy-D-galactitol, 35.2 nmol(2.3%) of 2-amino-2-deoxy-bglucose, and a small proportion of amino acids, and it was identified as oligosaccharide alditols with admixture of glycopeptides. The main portion of this fraction (0. i6pmol of amino alditols) was concentrated with addition of acetic acid in methanol (3 x 7 mL), the residue was dissolved in 5% acetic acid (I mL), and the solution was applied to a column (1 .I5 x 12cm) of Dowex 5OW-X2 (Ht) cation-exchange resin. The oligosaccharide alditols were eluted with 5O/oacetic (25 mL) at 5“, the column was washed with water (30mL), and glycopeptides were eluted with M NH,OH (60mL). The oligosaccharide fraction contained 0.155 pmol of 2-amino-2-deoxy-r>-galactitol and 17 nmol of 2-amino-2-deoxy-o-glucose. (c/.

Lithium horohpdride

treutnw~t

qf’ N gl!‘copeptide

,fktion

,fivm .fbtuirl or

asidofituin.

The glycopeptide fraction containing 1.2 jlrnol of 2-amino-2-deoxy-bglucose was treated with alkaline LiBH, for 5 h at 45 in 70% trrt-butyl alcohol (1.5 mL), and the mixture ofthe N-OS and glycopeptides was isolated by gel chromatography on a Sephadex G-l 5 column. as reported previously”. Further fractionation of the mixture prepared from asialofetuin was performed as described” and the N-OS were obtained in a yield of 55%. In the case of fetuin, the mixture of N--OS and glycopeptides was dissolved in 5% acetic acid (0.1 mL), and the solution was applied to a column (0.3 x 10 cm) of Dowex 5OW-X2 (H ‘) cation-exchange resin in 5% acetic acid at 5.. The N~-OS were eluted with 5% acetic acid. The first fraction (0.43 mL, 60% of the column volume) contained 0.48 pmol of 2-amino-2-deoxy-D-glucose and 18 nmol of 2-amino-2-deoxy-n-glucitol, and it was identified as a mixture of N--OS (34%) and N-OS alditols (7.5%). The second fraction (0.43 mL) contained 0.33 ~mol(28”/0) of 2-amino-2-deoxy-I>-glucose and some

SEQUENTIAL

SPLITTING

OF GLYCANS

FROM GLYCOPROTEINS

75

amino acids, and it was identified as a mixture (N 1: 1) of N-OS and N-glycopeptides. Treatment of GSG H and B. - (a). Analytical experiments. Solutions of GSG (1.5 mg) in reagent A, B, or C (0.5 mL) were incubated for 16 hat 50” (for A and B), or for 5 h, 10 h, and 16 h at 60” (for C). After cooling, water (1.5 mL) was added to each solution, which was acidified with acetic acid. The solution was concentrated with addition of methanol (3 x 3mL), and the residue was hydrolyzed and subjected to analysis for amino acids (Table II), 2-amino-2-deoxy-D-galactose, -n-glucose, and -n-galactitol. The yields of oligosaccharides are shown in Table I. (b). Isolation of oligosaccharides after treatment of GSG H with reagent C. An aqueous solution (1.4 mL) of GSG H (6 mg containing 2.5 pmol of 2-amino-2-deoxy-ngalactose) was mixed with water (0.2 mL), cadmium acetate-EDTA.Na, solution (0.29 mL), 0.5111 NaOH (0.11 mL), NaBH, (152 mg), and butanol(O.1 mL). The mixture was incubated for 16 h at 60”, cooled, and diluted with water (6 mL). The solution was acidified with acetic acid to pH 777.5 and diluted with water to 10mL. Half of the solution was acidified with acetic acid to pH 5-6 and concentrated with addition of methanol (3 x 5 mL). The residue was dissolved in 5% acetic, and the solution was applied to a column (1.15 x 14.5 cm) of Dowex 5OW-X2 (H+) cationexchange resin. The oligosaccharide fraction was eluted with 5% acetic acid (30mL), the column was washed with water (45 mL) to neutrality, and the glycopeptide fraction was eluted with M NH,OH (75 mL). The oligosaccharide fraction contained 1.04 pmol (83%) of 2-amino-2-deoxy-D-galactitol and 13 nmol (1%) of 2-amino-2-deoxy-ngalactose (the latter originated from the admixture of the glycopeptides). The glycopeptide fraction contained 50 nmol (4%) of 2-amino-2-deoxy-D-galactose and 29 nmol (2.3%) of 2-amino-2-deoxy-D-galactitol (from the admixture of the oligosaccharides due to N-deacetylation of 2-amino-2-deoxyhexoses). Loss of 2-amino-2-deoxy-n-galactose owing to irreversible adsorption of glycopeptides on Dowex resin was as much as 10%. The second half of the solution was concentrated to 2 mL and the solution was applied to a column (1.8 x 95 cm) of Sephadex G-l 5 which was eluted with 4mM NH,OH (pH N 9.5) to give two fractions. Fraction I (K,, < 0.27) contained amino acids (N 85%) and 2-amino-2-deoxy-D-galactose(O.l04pmol, 8.3%). Fraction II (Z&0.27-1) was concentrated with addition of acetic acid in methanol (3 x 7 mL), the residue was dissolved in 5% acetic acid (5mL), and the solution was applied on to a column (1.15 x 14.5 cm) of Dowex 5OW-X2 (H+) cation-exchanges resin. The oligosaccharide fraction was eluted with 5% acetic acid (30mL), the column was washed with water (45 mL), and the glycopeptide fraction was eluted with M NH,OH (75 mL). The oligosaccharide fraction contained 1.09 pmol(87.5%) of 2-amino-2-deoxy-D-galactitol, and the glycopeptide fraction 34 nmol(2.7%) of 2-amino-2-deoxy-D-galactitol and 21 nmol (1.7%) of 2-amino-2-deoxy-D-galactose. Treatment of ovumucoid - (a). With alkaline sodium borohydride and Cdz’ (reagent B). A solution (0.6 mL) of ovomucoid (3 mg containing 1.9pmol of2-amino-2deoxy-D-glucose) was mixed with water (O.l4mL), cadmium acetate-EDTA.Na, solution (O.l4mL), 0.5~ NaOH (O.l2mL), NaBH, (38 mg), and butanol (0.05mL). The mixture was incubated for 16 h at 50”, and then cooled. Water (1 mL) was added, and the solution was acidifield with acetic acid to pH 7 and it was applied onto a column

76

L.M. LIKHOSHERSTOV

(1.8 x 95 cm) of Sephadex

G-50 in hM

NH,OH

and two fractions

et d.

were obtained.

Fraction I (K,, < 0.45) contained most of the amino acids and 1.71 pmol (90%) of 2-amino-2-deoxy-n-glucose, and was identified as a mixture of high-molecular-weight glycopeptides.

Fraction

II (K,, 0.45-0.75)

contained

0.18 ,umol (9.5%) of 2-amino-2-

deoxy-D-glucose and 9.5 nmol(O.5%) of 2-amino-2-deoxy-D-glucitol, and was found to be a mixture of glycopeptides ( - 6%) and N-OS alditols (- 3%). (h j. With alkaline sodium borohydride (reagent A). The reaction was carried out in an analogous manner. After gel chromatography on Sephadex G-50. two fractions were obtained. Fraction I (K,, < 0.45) contained a small proportion of amino acids and 28 nmol (1.5%) of 2-amino-2-deoxy-n-glucose, and it was identified as a mixture of glycopeptides. Fraction II (K,, 0.4550.75) contained amino acids, 1.52pmol(80.3%) of 2-amino-2-deoxy-D-glucose, and 51.2 nmol(2.7%) of 2-amino-2-deoxy-D-glucitol, and it was found to be a mixture of glycopeptides ( - 67%) and N-OS alditols (- 17%). (c). With 0.05~ sodium hydroxide. The treatment was carried out in an analogous manner. After gel chromatography on Sephadex G-50, Fraction II (K,, 0.45-0.75) was concentrated to dryness, the residue was dissolved in 50mM NaOH (0.1 mL), and NaBH, (3 mg) was added. The mixture was incubated for 3 h at 40’ and then diluted with water to 0.5 mL. Acetic acid (0.15mL) was added and boric acid removed by coevaporation with methanol (3 x 2mL). Analysis of the residue with an amino acids analyzer revealed the presence of amino acids, 2-amino-2-deoxy-n-glucose (0.266 pmol, 14%), and 2-amino-2-deoxy-D-glucitol (13.3 nmol, 0.7%). mixture of glycopeptides (- 10%) and N-OS (_ 3.5%).

Thus,

Fraction

II is a

REFERENCES I J. B. L. Damm, J. P. Kamerling. G. W. K. van Dedem, and J. F. G. Vliegenthart. ClJwconjuyatr J., 4 (1987) 1299144. 2 S. A. M. Korrel, K. J. Clemetson. H. van Halbeek, J. P. Kamerling, J. J. Sixma. and J. F. G. Vliegenthart, Glw~onjuyute J., 2 (1985) 229~ 234. 3 M. L. Rasilo and 0. Renkonen. FEBS Le/r.. 135 (1981) 38 42. 4 E. F. Hounsell. N. J. Pickering, M. S. Stall. A. M. Lawson. and T. Feizi, Bioc~hmi. Sot. Trcw., I2 (1984) 607 610. S H. Debray, G. Strecker, and J. Montreuil, Biochcm. Sot. Truns., 12 (1984) 61 1~612. 6 L. M. Likhosherstov, 0. S. Novikova, V. A. Derevitskaya, and N. K. Kochetkov, &jkl. Akari. Nuuk SSSR, 274 (1984) 222-225. 7 T. Saton, S. Suzuki, Y. Miyaji, and Z. Imai, Tefrahrdron Lrrr., 52 (1969) 4555-4558. 8 T. Saton. N. Mitsuo. M. Nishiki, Y. Inoue, and Y. Ooi. Chwr. Pharm. &/I.. 29 (1981) 144331445. 9 T. Cioto and Y. Kishi. Tctruhdrlron Let/., I5 (1961) 513 515. 10. R. N. Iyer and D. M. Carlson, Adz. Biochem. Bioph~s.. 142 (1971) 101-105. 1I L. M. Likhosherstov, 0. S. Nov,ikova, V. A. Derevitskaya, and N. K. Kochetkov. Dokl. Akad. Nmk SSSR., 303 (1988) 641 645. 12. L. M. Likhosherstov, 0. S. Novikova, V. E. Piskarev, E. E. Trusikhina, V. A. Derevitskaya. and N. K. Kochetkov,. Corhol~wlr. Rex., I78 (1988) 155-l 63. 13 J. G. Davis, C. J. Mares, and J. W. Donovan, Biochemistr~~, IO (1971) 39-42. 14 L. M. Likhosherstov, V. A. Derevitskaya, and V. I. Fedorova, Biokhimia, 34 (1969) 4550. 15 L. M. Likhosherstov, N. P. Arbatsky. and V. A. Derevitskaya, Biokhimin. 38 (1973) 723- 726. I6 M. Yagushi and N. B. Perry, Cwt. J. Biochrm., 48 (1970) 386 388. 17 L. M. Likhosherstov, 0. S. Novikova. V. A. Derevitskaya, and N. K. Kochetkov, IX). Akad. Nrruk SSSR., Ser. Khim., (1986) 166331669.

A selective method for sequential splitting of O- and N-linked glycans from N,O-glycoproteins. - PDF Download Free (2024)

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